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Journal of Pharmaceutical and Biological Sciences


Isolation, identification, enumeration and antibiotic profiling of microbes from soil contaminated with hospital waste dumbing


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Author Details : C. Prakasam, E. Poongothai N. Siddharthan, N. Hemalatha

Volume : 5, Issue : 3, Year : 2017

Article Page : 126-133


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Abstract

Hospital waste is a potential health hazards to the health care workers and public acquired infection, transfusion transmitted diseases. Increasing land and water pollution lead to increasing possibility of contracting many diseases. Study has compelled the authorities to think seriously about hospital wastes and the disease transmitted through their improper disposal. Soil samples were collected from two different hospitals in Salem city. A total of six isolated were identified and purified from the samples, further screened for individual antibiotics at their respective concentrations and all the six isolates were found to be strong resistant against antibiotics selected in the study. In this present study, Pseudomonas sp only possessed a positive virulence characteristic such as haemolysis, protease, coagulase, lecithinase, pyocyanin and lipase when compare to other species like E.coli, Proteus, Bacillus, Klebsiella, Staphylococcus sp. These are all very low in prevalence of enzymes associated with bacterial pathogens.

Keywords: Hospital waste, Improper disposal, Antibiotic resistant, Virulence characteristics, Pseudomonas sp

How to cite : Prakasam C, Siddharthan E P N, Hemalatha N, Isolation, identification, enumeration and antibiotic profiling of microbes from soil contaminated with hospital waste dumbing. J Pharm Biol Sci 2017;5(3):126-133

Copyright © 2017 by author(s) and J Pharm Biol Sci. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC-BY-NC 4.0) (creativecommons.org)