Article Access statistics

Viewed: 948

Emailed: 0

PDF Downloaded: 322

Indian Journal of Pharmacy and Pharmacology


Development and Validation of RP-HPLC Method for Simultaneous Estimation of Bromhexine and Erythromycin in Bulk and Pharmaceutical Dosage Forms


Full Text PDF Share on Facebook Share on Twitter


Author Details : Mounika K, Lakshmana Rao A

Volume : 3, Issue : 2, Year : 2016

Article Page : 63-68


Suggest article by email


Abstract

A simple, rapid, accurate and precise isocratic reversed phase high performance liquid chromatographic method has been developed and validated for simultaneous estimation of Bromhexine and Erythromycin in tablet dosage forms. The chromatographic separation was carried out on Hypersil BDS C18 column (150 mm x 4.6 mm I.D., 5 µm particle size) with a mixture of 0.01N phosphate buffer and acetonitrile in the ratio of 35:65 v/v as a mobile phase at a flow rate of 1.0 mL/min. UV detection was performed at 224 nm. The retention times were 4.619 minutes and 2.329 minutes for Bromhexine and Erythromycin respectively. Calibration plots were linear (r2=0.999 for both Bromhexine and Erythromycin respectively) over the concentration range of 2-12 µg/mL for Bromhexine and 62.5-375 µg/mL for Erythromycin. The method was validated for linearity, precision, accuracy and robustness. The proposed method was successfully used for simultaneous estimation of Bromhexine and Erythromycin in tablet dosage forms. Validation studies revealed that the proposed method is specific, rapid, reliable and reproducible. The high % recovery and low % RSD values confirm the suitability of the proposed method for routine quality control analysis of Bromhexine and Erythromycin in bulk and tablet dosage forms.

Keywords:
Bromhexine, Erythromycin, Estimation, HPLC

How to cite : Mounika K, Lakshmana Rao A, Development and Validation of RP-HPLC Method for Simultaneous Estimation of Bromhexine and Erythromycin in Bulk and Pharmaceutical Dosage Forms. Indian J Pharm Pharmacol 2016;3(2):63-68

Copyright © 2016 by author(s) and Indian J Pharm Pharmacol. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC-BY-NC 4.0) (creativecommons.org)