Print ISSN:-2394-546X

Online ISSN:-2394-5478

CODEN : IJMR06

Current Issue

Year 2020

Volume: 7 , Issue: 2

Article Access statistics

Viewed: 518

Emailed: 0

PDF Downloaded: 166

Indian Journal of Microbiology Research


Detection of carbapenem resistant enterobacteriaecae and the comparison between phenotypic methods and multiplex PCR


Full Text PDF Share on Facebook Share on Twitter


Research Article

Author Details : Rubhana Qadri*, Suhail Ahmed, Dalip K Kakru, Gulnaz Bashir, Bashir Fomda

Volume : 6, Issue : 2, Year : 2019

Article Page : 126-130


Suggest article by email


Abstract

Introduction: Carbapenem resistant Enterobacteriacea (CRE) has created a remarkable health distress. Definitive detection of Carbapenemase producing CRE is the first step in combating this problem.
Objective: To detect and compare CP- CRE both by phenotypic and molecular methods.
Materials and Methods: A total of 52 carbapenem resistant clinical isolates were screened for the presence of carbapenemase genes by routine phenotypic methods like modified hodge test and combined disc test as well by multiplex PCR.
Results: Out of the total 52 meropenem resistant isolates, 35 were modified hodge test positive and 33 were combined disc test positive. 42 isolates were found harbouring one or more than one gene. blaKPC alone was present in 38 isolates, while as blaKPC with blaNDM were present in 1 isolate and blaKPC with blaIMP was seen in 1 isolate. blaNDM alone in 2 isoates, blaIMP and blaVIM alone in none of the isolates.
Conclusion: Accurate detection of carbapenemase producing genes by molecular methods overcomes the problem related to CRE. Though there is no signal method that is ideal for all situations.

Keywords: Modified hodge test, Combined disc test, Multiplex PCR, Carbapenem resistant Enterobacteriaecae.

Doi : 10.18231/j.ijmr.2019.027

How to cite : Qadri R, Ahmed S, Kakru D K, Bashir G, Fomda B, Detection of carbapenem resistant enterobacteriaecae and the comparison between phenotypic methods and multiplex PCR. Indian J Microbiol Res 2019;6(2):126-130

Copyright © 2019 by author(s) and Indian J Microbiol Res. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC-BY-NC 4.0) (creativecommons.org)