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IP Indian Journal of Conservative and Endodontics

Evaluation of nanoleakage following deproteinization of dentin – a confocal laser scanning microscopic study

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Author Details : S. Anita Rao, T. Muralidhar, Kirit Golla

Volume : 2, Issue : 2, Year : 2017

Article Page : 61-65

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Aim: This study evaluated the amount of nanoleakage following deproteinization of dentin with newer deproteinizing agents such as Ascorbic acid and Zinc Hydroxide.
Materials and Method: A total of 40 extracted human premolars were used in this study. Standardized Class V cavities were prepared on the buccal. The teeth were randomly divided into four groups (n= 10) based on the different agents used for deproteinization.
Group 1: Normal saline was used as a control.
Group 2: 3% NaOCl
Group 3: 10% Ascorbic acid
Group 4: 5% Zinc hydroxide
All the teeth were dried and dentin bonding agent was applied and light cured.
The restorative procedure was performed by inserting composite resin in three horizontal increments. Each increment was individually light cured for 20s from all the surfaces to ensure complete polymerization. For dye penetration the specimens were stored in 50% alcoholic solution of 1% wt Rhodamine B fluorescent dye for 24 hours. The teeth were then sectioned parallel to the long axis of the tooth using low speed diamond disc under copious water supply. The sectioned specimens were then polished using 600 grit silicon carbide paper. The dentin adhesive region was examined using Confocal Laser Scanning microscope.
Results: In group 3, when 10% ascorbic acid was used after acid conditioning of dentin, the confocal laser scanning microscopic images showed least amount of dye penetration thus indicating least nanoleakage. Least mean nanoleakage score was seen in Group 3.
Conclusion: Ascorbic Acid has least nanoleakage which was not significant from NaOCl. However both ascorbic acid and NaOCl had significantly lower nanoleakage than Zinc Hydroxide and Saline. Zinc hydroxide has also significantly lower nanoleakage than saline.
Clinical Significance: Deproteinization of dentin with newer biocompatible agents such as Ascorbic acid decreased the amount of nanoleakage when compared with routinely used agents such as sodium hypochlorite.

Deproteinization, Nanoleakage, Confocal laser scanning microscopy, Composite

How to cite : Rao S A, Muralidhar T, Golla K, Evaluation of nanoleakage following deproteinization of dentin – a confocal laser scanning microscopic study. IP Indian J Conserv Endod 2017;2(2):61-65

Copyright © 2017 by author(s) and IP Indian J Conserv Endod. This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International License (