Print ISSN:-2394-2118

Online ISSN:-2394-2126

CODEN : IJCAGX

Current Issue

Year 2020

Volume: 7 , Issue: 2

Article Access statistics

Viewed: 664

Emailed: 0

PDF Downloaded: 228

Indian Journal of Clinical Anatomy and Physiology


Biofilm formation and extended spectrum beta-lactamase production in Klebsiella pneumoniae isolates from respiratory samples in a tertiary care hospital


Full Text PDF Share on Facebook Share on Twitter


Author Details : Amar Sunil Lobo, MS. Moosabba, Rouchelle C. Tellis, Ronald Roche

Volume : 3, Issue : 1, Year : 2018

Article Page : 6-9


Suggest article by email


Abstract

Nosocomial and community acquired infections are being caused by Klebsiella pneumoniae (K. pneumoniae) worldwide. K. pneumoniae among gram negative organisms is an important bacterium responsible for site specific infection when they grow as biofilm. Survival of K. pneumoniae is facilitated by biofilm formation that makes it easier to multiply and spread to various sites. Extended–spectrum beta-lactamase (ESBL) producing K. pneumoniae isolates from respiratory samples were used to know the occurrence of biofilm formation conducted in a tertiary care medical college hospital in Mangalore, Dakshina Kannada District, Karnataka, India.
To know the occurrence of biofilm formation among extended–spectrum beta-lactamase (ESBL) producing K. pneumoniae isolates from respiratory samples a prospective study has been conducted in a tertiary care medical college hospital in Mangalore, Dakshina Kannada District, Karnataka, India. A total of 87 K. pneumoniae isolates from respiratory samples were characterized according to standard microbiological specific procedures. Screening was done for K. pneumonia Biofilm formation in terms of their antibiotic sensitivity pattern by using standard Kirby Bauer disc diffusion method and presumptive ESBL production by double disk synergy test (DDST). Out of 87 clinical isolates from respiratory samples, 45 (51.72%) were found to be biofilm producers. 29 (33.33%) isolates were ESBL producers and all them produced biofilm. ESBL forming K. pneumoniae isolates had a significantly greater capacity to form strong biofilm (72.4%) than non ESBL producing K. pneumoniae isolates (27.58%).

Keywords: Klebsiella pneumoniae, Nosocomial infections, ESBL production, Kirby-Bauer, Biofilm formation.

Doi : 10.18231/2456-9542.2018.0002

How to cite : Lobo A S, Moosabba M, Tellis R C, Roche R, Biofilm formation and extended spectrum beta-lactamase production in Klebsiella pneumoniae isolates from respiratory samples in a tertiary care hospital. Indian J Clin Anat Physiol 2018;3(1):6-9

Copyright © 2018 by author(s) and Indian J Clin Anat Physiol. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC-BY-NC 4.0) (creativecommons.org)