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Indian Journal of Clinical Anatomy and Physiology


EFFECT OF CAFFEINE ON FROG MUSCLE


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Author Details : Sanjay Kumar, Prakash Tomar, J. Prakasha Rao

Volume : 2, Issue : 2, Year : 2015

Article Page : 62-65


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Abstract

Aim: The aim of the present investigation is to study the effects of caffeine on frog skeletal, cardiac and smooth muscle tissues.

Material and methods: In this study muscle strips were obtained from Sartorius muscle, heart muscle and rectum of frog after pithing the animal. Caffeine (30mM) was administered in aqueous form with the help of Micropipette directly upon the isolated muscle sample. These isolated samples were prepared, fixed and analyzed with the help of laser beam where linear movement of muscle contraction is converted to angular movement which was recorded on the graph paper and further statistical analysis was done using student’s t-test to determine significance of differences between means. A “p” value of < 0.05 was considered significant.
Conclusion: Caffeine (30mM) induces sustained contraction in skeletal and smooth muscle, but cardiac muscle does not respond to caffeine though it increases the natural beat of sinoatrial preparation.

Keywords: Caffeine, laser beam, student’s t-test, pithing

How to cite : Kumar S, Tomar P, Rao J P, EFFECT OF CAFFEINE ON FROG MUSCLE. Indian J Clin Anat Physiol 2015;2(2):62-65

Copyright © 2015 by author(s) and Indian J Clin Anat Physiol. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC-BY-NC 4.0) (creativecommons.org)